StepOnePlus™ Real-Time PCR System

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Features of the StepOnePlus™ Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays

• Sensitive 4-color optical LED recording system

• Intuitive and robust software perfect for both first-time and advanced users

• Simple and flexible instrument set-up and usage

New StepOne™ Software

The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up

This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Sensitive LED-Based 4 Color Fluorescence Reading

The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Compatible with Many Genomic Analysis Techniques

Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.

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Technical Specification

• Calibrated Dye: VIC™, SYBR™ Green I, ROX™, JOE™, NED, FAM™, TAMRA™
• Capacity: 96 x 0.1 ml tubes, 12 x 8-tube strips, 1 x 96-well plate
• Detection Method: SYBR, Primer-Probe Detection
• Dimensions: 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
• Format: 96-well plate, 8-tube strips, 0.1 ml tubes
• Green Features: Sustainable packaging
• High-throughput Compatibility: Multiplexing
• Optics: 4 emission filters, Blue LED excitation source, photodiode
• Passive Reference Dye: ROX (Separate Tube), No ROX, ROX (Pre-mixed)
• Peak Block Ramp Rate: 4.6°C⁄sec
• Product Line: StepOnePlus™
• Reaction Speed: Fast, Standard
• Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
• Run Time: <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
• Sample Ramp Rate: Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
• Temperature Accuracy: 0.25°C (35 to 95°C) of display temperature
• Temperature Range (Metric): 4-100°C
• Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature
• Thermal Cycling System: Peltier-Based System
• Weight: 24 kg (53 lbs.)
• Display Type: LCD Touch Screen
• For Use With (Equipment): StepOnePlus™
• Includes: No Computer
• Dynamic Range: Linear Dynamic Range greater than 9 log units (detection)
• Sensitivity: 1 copy
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Description

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Features of the StepOnePlus™ Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays

• Sensitive 4-color optical LED recording system

• Intuitive and robust software perfect for both first-time and advanced users

• Simple and flexible instrument set-up and usage

New StepOne™ Software

The StepOne™ Software included with the StepOnePlus™ Real-Time PCR System runs on the Windows® XP operating system and provides instrument control, data collection, and data analysis capabilities (analysis software is also compatible with Windows® 7). This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

Rapid and Simple Assay Set-Up

This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Sensitive LED-Based 4 Color Fluorescence Reading

The StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

Compatible with Many Genomic Analysis Techniques

Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus™ Real-Time PCR System and StepOne™ Software.

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