QuantStudio™ 5 Dx Real-Time PCR System

The Applied Biosystems QuantStudio 5 Dx Real-Time PCR System is a CE-IVD marked quantitative PCR system that delivers proven performance and support to help meet the needs of clinical/hospital laboratories and test developers, in a compact footprint and cost-effective package.

Flexibility and security
The QuantStudio 5 Dx software is a combined-function software that enables both Test Development (DEV) and In Vitro Diagnostic (IVD) operation as discrete operating modes, minimizing risk of operation in the incorrect instance.

The software enables you to maintain secure access to the instrument, specify user access to various software functions, record all actions completed by users, and sign data electronically by virtue of Security, Auditing, and E-signature features.

This QuantStudio 5 Dx system is offered in a 96-well, 0.2 mL configuration and accommodates reaction volumes from 10 to 100 uL, as well as both standard and fast chemistries to meet varying user demands.

Trusted performance
Resulting from over 20 years of real-time PCR experience, the QuantStudio 5 Dx system includes proven Applied Biosystems OptiFlex technology and VeriFlex blocks for enhanced data quality. By enabling de-coupling of excitation and emission filters with OptiFlex technology, users may optimize performance for specific dyes and when multiplexing. With VeriFlex blocks, users may precisely set the temperature on six independently-controlled thermal zones across the 96-well sample block for optimal PCR amplification performance.

Cost effective
The QuantStudio 5 Dx system is our most affordable CE-IVD qPCR platform. It is factory-calibrated and, following installation qualification and operational qualification by a factory-certified engineer, ready for operation by trained users. Subsequent CE-IVD dye calibrations are now user-enabled for convenience and to minimize ongoing operating costs.

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Technical Specification

• Reaction volume : 96-well, 0.2 mL block: 10–100 μL
• Footprint: (W x D x H) 27 x 50 x 40 cm
• Weight: 27 kg
• Excitation source: Bright-white LED
• Optical detection: 6 decoupled filters, CMOS camera
• Excitation/detection range :450–680 nm/500–730 nm
• Temperature range: 4–99.9°C
• Maximum block ramp rate: 6.5°C/sec
• Average sample ramp rate: 3.66°C/sec
• Temperature uniformity: 0.5°C
• Temperature accuracy: 0.25°C
• Heating and cooling method: Peltier
• Independent temperature zones: 6 VeriFlex™ zones (5°C zone to zone)
• Chemistries: Both fast and standard
• Run times :<30 min (fast mode)
• Validated reaction volumes: 10–100 µL
• Factory-calibrated dyes: FAM™, SYBR™, VIC™, ABY™, NED™, TAMRA™, JUN™, ROX™, Mustang Purple™, and Cy®5 dyes
• Multiplex: 5-plex with 1 passive reference; 6-plex with no passive reference
• Sensitivity: 1 copy; detect differences as small as 1.5-fold in target quantities in singleplex
• Dynamic range: 9 logarithmic units of linear dynamic range
• Security and auditing features: Integrated tools to assist with 21 CFR Part 11 compliance

Research Use Only

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Description

The Applied Biosystems QuantStudio 5 Dx Real-Time PCR System is a CE-IVD marked quantitative PCR system that delivers proven performance and support to help meet the needs of clinical/hospital laboratories and test developers, in a compact footprint and cost-effective package.

Flexibility and security
The QuantStudio 5 Dx software is a combined-function software that enables both Test Development (DEV) and In Vitro Diagnostic (IVD) operation as discrete operating modes, minimizing risk of operation in the incorrect instance.

The software enables you to maintain secure access to the instrument, specify user access to various software functions, record all actions completed by users, and sign data electronically by virtue of Security, Auditing, and E-signature features.

This QuantStudio 5 Dx system is offered in a 96-well, 0.2 mL configuration and accommodates reaction volumes from 10 to 100 uL, as well as both standard and fast chemistries to meet varying user demands.

Trusted performance
Resulting from over 20 years of real-time PCR experience, the QuantStudio 5 Dx system includes proven Applied Biosystems OptiFlex technology and VeriFlex blocks for enhanced data quality. By enabling de-coupling of excitation and emission filters with OptiFlex technology, users may optimize performance for specific dyes and when multiplexing. With VeriFlex blocks, users may precisely set the temperature on six independently-controlled thermal zones across the 96-well sample block for optimal PCR amplification performance.

Cost effective
The QuantStudio 5 Dx system is our most affordable CE-IVD qPCR platform. It is factory-calibrated and, following installation qualification and operational qualification by a factory-certified engineer, ready for operation by trained users. Subsequent CE-IVD dye calibrations are now user-enabled for convenience and to minimize ongoing operating costs.

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